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Description
RBX1 Recombinant Rabbit mAb (S-R355)Product Specification Host Rabbit Synonyms E3 ubiquitin protein ligase RBX1, E3 ubiquitin protein transferase RBX1, Protein ZYP, RING finger protein 75, RING box protein 1, Regulator of cullins 1, RNF75, ROC1 Location Cytoplasm, Nucleus Accession P62877 Clone Number S R355 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, ICC, ICFCM Reactivity Hu, Ms, Rt Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical
Product Specification
| Host | Rabbit |
| Synonyms | E3 ubiquitin-protein ligase RBX1, E3 ubiquitin-protein transferase RBX1, Protein ZYP, RING finger protein 75, RING-box protein 1, Regulator of cullins 1, RNF75, ROC1 |
| Location | Cytoplasm, Nucleus |
| Accession | P62877 |
| Clone Number | S-R355 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| ICC | 1:500 | null |
| ICFCM | 1:500 | null |
Background
RBX1 is an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over.
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4.0 ★★★★★
Based on 28 reviews
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Product Reviews
★★★★★ 5
wonderful trans sapphic shifter romance
Format: Kindle
When life falls apart, perhaps taking a break is just what is needed. But when Natalie (said trans woman) and Wren (loner alpha wolf) first see each other, it seems that perhaps that there might be something between them, but that just makes everything a lot more complicated. After all, a bond between a human and a wolf isn't a thing, is it?
This was a wonderful read and I absolutely loved this story. Yes, while I may have a strong like of shifter stories, that also means that I also have expectations, and this story most definitely exceeded them.
The trans representation was great. Natalie's struggles for acceptance, both internal and external resonating, and the difficulties that she's been through definitely resonate and are far too common of trans people. She's not a strong confident lead, but a woman who has issues and who does need the help and strength of others. She leans on others for better or for worse, but that makes her more real, and seeing her grow and become more confident as the story progresses is heart warming and wonderful. And the development of the bond - very nice!
Wren is just as much a flawed character, with what's she's been through, and the knowing of the knowledge that she lacks. She starts off strong, as one would expect from a werewolf, but she also grows, becoming more than what she was in the beginning as well.
For all of the angst and mistakes that are made, this is a story of growth and acceptance, of being able to gain what you actually desire, and to be able to embrace the support of others, knowing that you aren't alone in the world.
It is a tale of finding love, of having a bond form, and being able to take it to the next level.
This really is a wonderful sapphic shifter story, and one that I would highly recommend.
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Reviewed in the United States on March 15, 2023
★★★★★ 4
Wow!
Format: Kindle
Natalie desperately needs a change to get over her messy breakup. Wren is a lone wolf. She is an Alpha and has no pack to run with. With so many things at stake, will their affair end in disaster or have they found what they’ve been looking for?
I loved this fresh take on shifters with the variety of shapeshifters, the dangerous hunt and the great romance. It made this story a fantastic read and an emotional rollercoaster.
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Reviewed in the United States on March 27, 2023
★★★★★ 5
Great shifter story with unique twist
Format: Kindle
The story and characters were great. It was great to finally find a sapphic story with a trans character who was pre-op or in this case just no-op. So many authors don't want to have a trans character unless she's had surgery, like another woman won't want you if you have your original parts. Thank you so much Mrs. Abbott for the trans representation on this front. Nat and Wren are wonderful together, and I love how Wren does her best to make sure Nat doesn't ever feel less than or wrong for being herself. I am curious how being a shifter will effect Nat's transition moving forward. Hopefully there is another book and it touches on it a little.
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Reviewed in the United States on June 28, 2023
★★★★★ 5
Couldn’t put it down!
Format: Kindle
I LOVED this book. I was sad when I finished it because every time I took a break, I knew i had something to look forward to. If that makes sense.
Also it needs to be said that I had grown a little tired of werewolf novels until I saw this one had a trans main character. I’m queer + cis and firmly believe even the driest crustiest tropes are fresh and new when they’re given to us by talented and passionate underrepresented voices. This book is a good example of that. It’s just so fun and hammers home the connection so many queer people feel towards monsters, the secret-keeping and the ostracism and the found family. I kept saying, “of course our human protagonist is taking to the werewolf life like a duck to water!“
BEING TRANS is as big a part of this narrative as BEING A WEREWOLF and it’s delivered to us with great skill. it is a fresh, fun, cool, tense, exciting, and sexy read.
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Reviewed in the United States on September 21, 2023
★★★★★ 4
unconditional love and miscommunication
Format: Kindle
Two women from vastly different backgrounds but equally toxic meet in instant connection. One is an alpha that doesn’t really know anything about being an alpha. The other is a trans woman that is struggling with confidence.
This story is engaging from beginning to end. Well written and the connection and story are very satisfying! The love and acceptance freely given (although often misperceived) is beautiful.
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Reviewed in the United States on June 17, 2023