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Description
DPD ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 200 ng/mL). Then dilute to the following concentrations: 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 200ng/mL standard working solution into the first EP tube and mix thoroughly to make a 100ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotin-Antibody Working Solution: Centrifuge the concentrated Biotin-Antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated Biotin-Antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent) and use on the same day. 4. Preparation of Enzyme Conjugate Working Solution: 15 minutes before use, centrifuge the 100x concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100x concentrated HRP enzyme conjugate to a 1x working concentration with universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Use the same day. 5. Preparation of 1x Wash Solution: Dispense 10mL of 20x Wash Solution into 190mL of distilled water (Concentrated Wash Solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return them to 4°C. 2. Sample Addition: Add 50 μL of sample or standard of varying concentrations to the corresponding wells. Add 50 μL of universal diluent to the blank wells, followed by 50 μL of Biotin-Antibody Working Solution to each well. Cover with film and incubate at 37°C for 1 hour. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate to minimize matrix effects. The sample concentration should be multiplied by the dilution factor when calculating the final concentration. It is recommended to run replicates for all samples and standards.) 3. Plate Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, then shake off the wash solution and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 4. Enzyme Conjugate Working Solution: Add 100 μL of enzyme conjugate working solution to each well. Cover with film and incubate at 37°C for 30 minutes. 5. Wash: Discard the liquid and wash the plate five times as in step 3. 6. Add substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 7. Add stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculation of experimental results: Result evaluation: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction value. Plot a standard curve for the four-parameter logistic function on double-logarithmic graph paper with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a competitive enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled antibody, and HRP conjugate are sequentially added to microwells pre-coated with a universal deoxypyridinol/deoxypyridinoline (DPD) antigen. After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the universal deoxypyridinol/deoxypyridinoline (DPD) content in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | All | |||||||||||||||||||||||||||||||||
| Synonym | General Deoxypyridinol;DeoxypyridinolineELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Deoxypyridinoline (DPD), also known as D-pyrilinks, Pyrilinks-D, or deoxyPYD, is one of two pyridine crosslinks that provide structural rigidity to type I collagen in bone. It is measured in urine tests and used along with other bone markers such as alkaline phosphatase, osteocalcin, and N-terminal telopeptide to diagnose bone diseases such as postmenopausal osteoporosis, bone metastases, and Paget's disease. It is also useful in monitoring treatment with bone-active medications such as estrogens and bisphosphonates. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 3.12-200ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.5 ★★★★★
Based on 22 reviews
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Product Reviews
★★★★★ 5
Keeps things organized
Color: Clear
These are worth buying. They can store so many things. We are using it for our vanities, and they work amazingly. They are super sturdy, and the clear plastic gives them a clean look. These are very helpful to have.
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Reviewed in the United States on April 5, 2026
★★★★★ 5
nice set
Color: Clear Black
I bought this set to organize a messy desk drawer and it made a huge difference right away. Having multiple sizes in one pack is what really makes this set useful. I was able to customize the layout instead of trying to force everything into a few fixed compartments.
The trays feel sturdy enough for everyday use and the clear design makes it easy to see everything at a glance. I used them for things like pens, paper clips, chargers, and random small items that usually get lost, and now everything actually has a place.
They also don’t slide around much once placed, especially if the drawer is fairly full, which I appreciated. Setup took just a few minutes and instantly made the space look cleaner and more put together.
If I had to note anything, the plastic is lightweight, so it is not heavy duty, but for organizing office supplies it works perfectly fine.
Overall, a really practical set that helps you stay organized without overthinking it.
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Reviewed in the United States on April 14, 2026
★★★★★ 4
Great organizers!
Color: Clear
This is a really practical organizer set. I used it in my bathroom and nightstand drawers, and it helped clean everything up fast.
The different sizes are the best part—you can mix and match depending on what you’re storing.
Only reason I’m giving 4 stars is because the plastic isn’t super heavy-duty, but it’s still perfectly fine for everyday use.
Good value for the amount you get.
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Reviewed in the United States on April 8, 2026
★★★★★ 5
Nice drawer organizers with a lot of versatility.
Color: Clear
I bought these to organize my desk drawer because it seems like I can never find anything I'm looking for without taking everything out first,which is always a pain,so I thought why not try a set of drawer organizers.Talk about night and day,these things keep all my stuff tidy and easy to find now,it's great!
The plastic doesn't feel cheap either and the different sizes are very versatile,they also come with enough rubber feet for all the containers,though you do have to peel and stick them on yourself,but it's easy and the adhesive feels very strong.
I would definitely recommend these if you're looking for some great containers to get things more organized.
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Reviewed in the United States on February 23, 2026
★★★★★ 5
Easy to configure. So helpful!
Color: Clear, Color: Clear
My house has old bathroom cabinets that are too narrow for most organizers I have found. These organizers come in different sizes so I can mix and match the pieces to fit the drawers and items I have to store.
I have filled two drawers with these and still have more containers to use. These are truly the tool for the job. They are sturdy, stable and I thought I would have trouble with them moving around but they stay in place while maintaining their versatility. If I need to switch out stuff, I just reconfigure the drawer. No assembly required.
One of my most favorite simple purchases. I can’t believe I have lived without these simple but helpful organizers!
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Reviewed in the United States on March 8, 2026