Pay in installments of $30.75 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 17 - Aug 22
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Caspase 2 Activity Detection KitProduct Specification Usage Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12. 5, 6. 25, 3. 125 M. Set the zero tube without pNA. 2. Take 100l of each concentration and add it to a 96 well plate or 100l cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x axis, and the corresponding pNA concentration is the y axis.
Product Specification
| Usage | Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12.5, 6.25, 3.125 μM. Set the zero tube without pNA. 2. Take 100μl of each concentration and add it to a 96-well plate or 100μl cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x-axis, and the corresponding pNA concentration is the y-axis. Use Excel to make a standard curve of pNA concentration versus OD405 value. Detection of Caspase 2 enzyme activity: 1. Take out an appropriate amount of Ac-VDQQD-pNA (2mM) and place it on an ice bath for later use. 2. According to the 100ul reaction system: blank control group: 40μl of detection buffer, 0μl of sample to be tested, 50μl of lysate, 10μl of Ac-VDQQD-pNA (2mM); Sample group: 40μl of detection buffer, 50μl of sample to be tested, 0μl of lysate, 10μl3 of Ac-VDQQD-pNA (2mM). Add Ac-VDQQD-pNA (2mM) and mix well. Be careful to avoid bubbles during mixing. Incubate at 37 º C for 60-120 minutes. A405 can be measured when the color change is obvious. If the color change is not obvious, the incubation time can be appropriately extended, or even overnight. 4. The A405 of the sample minus the A405 of the blank control is the absorbance produced by pNA catalyzed by caspase 2 in the sample. By comparing the standard curves obtained in the above steps, it is possible to calculate the amount of pNA catalytically produced in the sample. |
| Theory | Based on the caspase 2 specific hydrolysis of peptide substrate Ac-VDQQD-pNA (Val Asp Val Ala Asp p nitroanilide), the released free nitroaniline pNA has a maximum absorbance at 405nm. Thus, the activity of caspase 2 can be detected by absorbance. Suitable for detecting Caspase-2 activity in mammalian tissues and cells. |
| Experiment Reagent Instrument | Microplate reader or spectrophotometer |
| Synonym | ch-1,Nedd-2,caspase-2,caspase 2 |
| Detection Type | Mammalian tissues, cells |
| Description | Caspase 2 activity detection kit is a kit that detects caspase 2 enzyme activity or purified caspase 2 enzyme activity in cell or tissue lysates by spectrophotometry. Caspases are a family of proteases that play an important role in the process of apoptosis. Caspase 2 can be activated during signal transduction of apoptosis. Caspase 2 mRNA can be cleaved into two different length forms. The full-length mRNA product of Caspase 2 can promote apoptosis, while the short mRNA protein product can inhibit apoptosis. In vitro, caspase 2 can be activated by caspase 1, caspase 3, and granzyme B. |
| Storage Temp. | When stored at-20 °C, Ac-VDQQD-pNA and pNA need to be stored in the dark. |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.3 ★★★★★
Based on 6 reviews
Sort
Product Reviews
★★★★★ 5
Just lovely
Size: 8.5" x 5.8" x 1.9", Color: Orange
Purchased this several months ago and liked it, but I had received so many complements on this tray since then I had to submit this review. The tray is sturdy and put together well, and the size is exactly what was given before purchase.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 27, 2026
★★★★★ 5
A Stylish and Functional Addition to My Décor!
Color: Gray 1 Pack, Size: 8" x 8" x 1.8", Color: Gray 1 Pack, Size: 8" x 8" x 1.8"
I recently purchased the ZHEM Valet Tray for Men in Dark Gray, and I couldn't be happier with this product. It's become an indispensable part of my daily routine and home décor.
The design and craftsmanship of this tray are outstanding. The dark gray color is elegant and complements my furniture perfectly. It's not just a valet tray; it's also a decorative piece that adds a touch of sophistication to my nightstand and dresser.
The size (8x8x1.75 inches) is ideal for holding my daily essentials like my watch, perfume, keys, and even napkins. I appreciate the versatility - it serves as a decorative piece, an organizer, and a functional tray all in one!
The quality is top-notch, and it feels sturdy and well-made. It's also easy to clean, which is a big plus.
Overall, the Tray has exceeded my expectations. It's a stylish and functional addition to my décor, and I highly recommend it to anyone looking for a versatile and attractive tray for their home. Five stars! ⭐⭐⭐⭐⭐
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 8, 2023
★★★★★ 5
Super cute landing space for keys
Color: White 1 Pack, Size: 8" x 8" x 1.8", Color: White 1 Pack, Size: 8" x 8" x 1.8"
I always have trouble finding my keys and so it’s been very helpful to have a dedicated spot to easily throw them in when I get home. At first I thought it would be too big but it’s the perfect size for two wallets and keys, and it’s good quality albeit a bit expensive. The gold isn’t too shiny either, it’s a nice touch and it really reduces the look of clutter on your surfaces and helps it look more organized. It also doesn’t slip around.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 2, 2026
★★★★★ 5
Nice tray
Color: Black 1 Pack, Size: 8" x 11" x 1.8"
Using it in the coffee table for remote contrls and headphone. Keeps organized and looks good. Perfect size.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 25, 2026
★★★★★ 5
Very nice
Color: White 1 Pack, Size: 8" x 8" x 1.8"
Very nice! It organized stuff in my moms room to look more put together.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 3, 2026