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Description
Human IL-12 p70 ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human IL 12 p70 antibodies were pre coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate. After incubation, IL 12 p70 present in the sample binds to the solid phase antibody
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-12 p70 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate. After incubation, IL-12 p70 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | Human interleukin 12p70 detection kit, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NKSF1, p35 | |||||||||||||||||||||||||||
| Composition |
Please use within the expiration date of the kit
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| Background | Interleukin 12 p70 (IL-12), also known as natural killer cell stimulating factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine consisting of a 40kDa (p40) subunit and a 35kDa (p35) subunit. Human p40 may circulate as a monomer, a homodimer, or a complex that binds to p35 to form IL-12 or binds to p19 to form IL-23. Both homodimers p40 and IL-23 can bind to IL-12R as non-signal antagonists. Alternatively, p40 can also bind to Rβ1, activating microglia and macrophages. The IL-12 p40 subunit shares with another heterodimeric cytokine, IL-23, with similar and distinct biological activity from IL-12. Cells known to produce IL-12 include macrophages and dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, plasmacytoid dendritic cells, microglia, CD8 + DCs (mouse cells only), and non-germinal center (CD38-CD44 +) B cells (human cells only). Functional IL-12 was shown to enhance cytotoxic activity and induce interferon gamma (IFN-gamma) production within NK cells, T cells, and dendritic epidermal T cells. IL12 has also been reported to induce IFN-γ production by macrophages. IL-12, together with other members of the IL-12 family, IL-23 and IL-27, promotes the development of CD4+Th1 immune response. In response to infection, IL-27 is first released, promoting the transition from Th0 to Th0/1. IL-12 is subsequently produced, resulting in Th1 effector cells. IL-12 combines with IL-18 to generate Th1 memory cells from effector cells, which are subsequently activated by IL-23. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 31.3pg/mL-2000pg/mL |
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★★★★★ 5
Foundation Piece of the puzzle
Format: Kindle
This book put everything into an organized way to understand what we are and what we can do as human beings. What a treasure to find.
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Reviewed in the United States on August 21, 2025
★★★★★ 5
Excelente autor.
Format: Paperback
Excelente y muy buen precio.
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Reviewed in the United States on May 26, 2026
★★★★★ 4
Good info.
Format: Paperback
Very interesting
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Reviewed in the United States on January 9, 2021
★★★★★ 5
Robert Monroe's Second Book on OBEs
Format: Paperback
In his second book, Robert Monroe continues to describe his personal experiences with Out-Of-Body travel. By the time he wrote this book, Mr. Monroe had traveled out-of-body so often that he is able to offer almost a "map" of the astral world and it's various levels and inhabitants. Mr. Monroe describes his fascinating encounters with a number of nonphysical beings, human and otherwise. He does not present himself as a religious person, but rather as a deeply curious individual in search of answers to questions as old as time. He attempts to make sense of what happens to us after we die and equally important, why we are here in the first place. The answers to these questions turn out to be complex and anyone looking for a simple explanation based on their own religious beliefs or upbringing may be disappointed, if not disturbed. Also, by this time, he had established much of his research techniques using hemi-sync, which he describes in some detail in the book.
This book is incredibly interesting and enlightening. My only complaint with the book is that Monroe often does not offer enough explanation or detail and left me with many unanswered questions. Each of Monroe's books is riveting on its own terms. However, I found that by reading the three books in order I was better able to follow Monroe's evolving understanding of the "other side".
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Reviewed in the United States on October 12, 2012
★★★★★ 5
One of the greatest books I’ve ever read
Format: Audiobook
I wish I could have met this man. I began having OBE’s about 8 years before I read the journeys trilogy. When he talked about the out of body simulations my head nearly popped off. I’ve had a few of those and never found them described in detail anywhere else. This is the greatest book and most profound trilogy on personal spiritual exploration and out of body travel ever written. Period. Such a gem and so incredible to be experienced outside the traditional contexts of yoga and meditation. A true original work. Thank you Bob. These 3 books changed my life and made me finally enquire “what is under the hood” in an OBE. What transports the astral body or consciousness from one dimension to another?
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Reviewed in the United States on January 6, 2023