Human BAFF/BlyS/TNFSF13B ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti human BAFF capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. BAFF in the sample binds to the solid-phase antibody. After thorough washing to remove unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After thorough washing again, TMB chromogenic substrate was added to avoid light for color development. The depth of color response has a positive correlation with the concentration of BAFF in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value under the condition of 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, BAFF detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.23pg/ml

Detection Range: 31.3-2000 pg/ml

Recovery Range: 80-121%

Storage Method: 2-8 ℃

Standard Curve:

Background:

B cell activating factor (BAFF) is also known as B lymphocyte stimulating factor (BLYS) or tumor necrosis factor ligand superfamily member 13b (TNFSF13B), which is expressed in B cell line cells, and as an effective B cell activator, plays an important role in the proliferation and differentiation of B cells. BAFF, as an immunostimulant, is necessary to maintain normal immunity. Insufficient BAFF expression will fail to activate B cells to produce sufficient immunoglobulin and lead to immunodeficiency. Overexpression of BAFF can cause excessive antibody secretion, leading to systemic lupus erythematosus, rheumatoid arthritis and other autoimmune diseases.