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Description
Organotial kidney organ class training kitProduct Specification Description Composition: Component name Specification Human kidney cancer organoid medium A 100mL Primary culture buffer B 250mL Human kidney cancer primary tissue digestive juice C 30mL Organoid passage digestive juice D 30mL Tissue preservation solution E 100mL Organoid cryopreservation fluid F 20mL Organoid subculture buffer G 250mL Instructions 1, primary (1) tissue after sampling suggested 2 8 The tissues were stored under C
Product Specification
| Description |
Composition:
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| Instructions |
1, primary (1) tissue after sampling suggested 2-8° The tissues were stored under C conditions and quickly transferred to a clean laboratory for tissue processing and cell separation. The information was photographed and registered. (2) Several culture dishes were prepared and primary culture buffer B, precooled at 4 ° C, was added for later use. (3) The sampling bottle was disinfected, and the tissue was placed in the Petri dish. After washing three times with primary culture buffer B, the tissue was cut into tissue blocks of about 1-3mm3in volume using ophthalmic scissors or scalpel. (4) The tissues were digested with human renal cancer primary tissue digestion solution C and shaken at 37℃ for 15-25min (the digestion was observed at any time during the digestion process). (5) A small amount of liquid was observed under the microscope. When more single cells or cell clusters below 70um were observed under the microscope, triploid volume primary culture buffer B was added to terminate the digestion. (6) The filtrate was filtered by a sieve with a pore size of 100um. After collecting the filtrate, the supernatant was removed after enrichment and centrifugation at 300g for 5min. The supernatant was resuspended by adding primary culture buffer B, and the supernatant was removed after enrichment and centrifugation at 300g for 5min. (7) The cell precipitate contained red blood cells, the supernatant was discarded, 1-2 ml of red blood cell lysate was added for 1-2min, diluted to 10mL, and centrifuged at 300g for 5min. (8) Matrix glue calculation: The collected tissue volume was observed after step 6, and the paving plate was resuspended by adding matrix glue (abs9495) at 25 times the tissue volume. (9) For the example of 24-well cell culture plate, 25uL-30uL tissue matrix glue mixture was dispensed to each well for paving (Note: matrix glue was maintained at 0-4 ° C for operation). (10) The paved culture plate was placed in an incubator at 37 ° C for 10-15min. After the matrix gel solidified, 500-750uL of human renal cancer organoid medium A (returned to room temperature) was added for culture. 2, Organoid subculture (1) The medium was aspirated by pipetting gun, and 1-2mL of 4 ° C organoid subculture buffer G was added to each well for 2min. (2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube, and allowed to rest for 10-15min at 4 ° C. (every 6-8 Wells are a group) (3) a: When the number of organoids is insufficient or the volume is small: Discard the supernatant by centrifugation at 300g for 5min, add 1mL organoid passage culture buffer G, resuspension and transfer into 1.5mL centrifuge tube, and discard the liquid by centrifugation at 300g for 5min for the fourth step. b: When organoids are large in number or size: Centrifugation at 300g for 5min to discard the supernatant, add 1-2mL organoid passage digestion solution D for 2-4min, add 3 times the volume of organoid passage culture buffer G to terminate digestion, centrifugation at 300g for 5min to discard the supernatant, add an appropriate amount of organoid passage culture buffer G to resuspend and transfer to a 1.5mL centrifuge tube. The supernatant was discarded by centrifugation at 300g for 5min, and the fourth step was carried out. (4) After organoids were collected, they were resuspended by adding matrigel, and 25uL of Matrigel per well was spread in 24-well cell culture plates and placed in an incubator for 10-15min. After the Matrigel had set, 500 to 750uL of human renal cancer organoid medium A was added to each well. 3, organoids were frozen (1) The medium was removed by suction with a pipetting gun, and 1-2 ml of 4 ° C organoid passage culture buffer G was added to each well and placed for 2min. (2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) (3) The supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended again, and the liquid was discarded by centrifugation at 300g for 5min. (4) Add 2mL organoid cryopreservation solution F to every 3 Wells, gently blow and mix, and transfer to cell cryopreservation tubes, 1mL per tube. (5) Make the labeling information, after the program cooling, move to -80° C refrigerator, 48h later, transferred to liquid nitrogen for long-term storage. Or put it into the 4℃ refrigerator for 40min, put it into the -20℃ refrigerator for 2h, move it to the -80℃ refrigerator, and put it into the liquid nitrogen tank for storage after 48h. 4, organoid resuscitation (1) 10mL organoid subculture buffer G was placed in a 15mL centrifuge tube. (2) The frozen organoid cells were removed from the liquid nitrogen tank and quickly thawed in a 37℃ water bath. (3) During the water bath melting process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely melted in a short time. (4) The dissolved organoid cells were quickly transferred to a 15mL centrifuge tube, gently blown with a pipette for 6-8 times, centrifuged at 300g for 5min, and then the supernatant was removed and the organoid cell precipitate was collected. An appropriate amount of organoid passage culture buffer G was added and resuspended, then transferred to a 1.5mL centrifuge tube and centrifuged at 300g for 5min, and the supernatant was discarded. (5) Add 100ul of Matrigel to each tube of cryopreserved tube and resuspend, 25uL of Matrigel in each well was spread in 24-well cell culture plate, placed in the incubator for 10-15min to gel, and 500-750uL of human renal cancer organoid medium A was added. |
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| Storage Temp. | Stored at 4 ° C, valid for 3 months; Stored at -20 ° C, valid for 1 year, see label for details. |
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4.9 ★★★★★
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Product Reviews
★★★★★ 5
ABSOLUTELY PERFECT - I LOVE THIS!!!
Color: Pink
I absolutely LOVE this case and the keyboard - it’s absolutely perfect in just about every way. If I had to get nit-picky and find something to give feedback on, I would say it is a bit heavy and it is a bit annoying that you have to move the keyboard forward when you want to use it and backward when you want to close up the case but I don’t care, I love it!
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Reviewed in the United States on March 12, 2026
★★★★★ 5
iPad case
Color: Navy Blue
Great case, functions well and the keyboard is a nice touch. Would recommend.
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Reviewed in the United States on June 3, 2026
★★★★★ 5
Quality Case
Color: Pink
This is an All in Pne case. I really like the lavender color with sparkles. It is a little heavier than expected but I still love it!
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Reviewed in the United States on March 18, 2026
★★★★★ 3
Ok, but don’t use hand cream
Color: Black, Color: Black
I like the case for its ability to house the keyboard, the pencil and the iPad in one convenient place. I find that the magnetic closure and magnets holding the keyboard are not as strong as a previous case I had purchased. The keyboard is great, I like the touchpad and the backlight colors. The cover material is not hand lotion friendly! I’ve tried water and lens cleaning wipes, and neither gets this cover clean. I hesitate to use any chemicals because the material is the type that will get sticky and nasty, and I don’t like that. I’ve included a photo of what the cover looks like after using a lens cleaning wipe. If the magnets were stronger and the cover material easier to clean, I would buy again.
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Reviewed in the United States on February 27, 2026
★★★★★ 4
great case!
Color: Navy Blue
pros; the color is beautiful. I picked the navy blue. It is exactly as the description described. It goes very well with my blue iPad. The size is very precise and fits perfectly on my iPad. The magnet that holds the case together is very strong. The iPad itself can pivot vertically and horizontally using that magnet. This works very smooth. The magnet closure for the case itself is strong and holds well. The magnet that holds the keyboard in place also works very well. Cons; when the keyboard is in its correct position, it is difficult to angle the iPad in front into the provided grooves. The keyboard gets into the way. This prevents the iPad from reaching the grooves provided. The keyboard feels very light and cheap.
The keyboard itself connected with the Bluetooth very easily. It works wonderful. I love the back light on the keyboard. The color of the keyboard is wonderful. However, as I said above, it does feel a bit cheap. But for 20 bucks, what can you ask for.
When the case is folded together with the keyboard in the iPad, it is a bit bulky. But I knew that from the previous reviews and was expecting it.
What I love most about this case is that the iPad can be removed using the magnet on the back from the keyboard portion, but it is still protected by the exterior plastic case. So in situations where I don’t need to use my keyboard, I can remove the iPad itself and know that it’s still protected overall I recommend this product.
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Reviewed in the United States on January 18, 2026