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Description
GAPDH Recombinant Rabbit mAb (S-240-147)Product Specification Host Rabbit Antigen GAPDH Synonyms Glyceraldehyde 3 phosphate dehydrogenase, Peptidyl cysteine S nitrosylase GAPDH Immunogen Synthetic Peptide Location Cytoplasm, Nucleus, Membrane Accession P04406P16858 Clone Number S 240 147 Antibody Type Rabbit mAb Application WB, IHC P, ICC Reactivity Hu, Ms, Rt, Zf Predicted Reactivity Hamster (95%), Mink (95%), Pig (95%), Squirrel (95%),Dog (95%), Cat (95%), Sheep (95%), Quail (95%), Bovine
Product Specification
| Host | Rabbit |
| Antigen | GAPDH |
| Synonyms | Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Membrane |
| Accession | P04406、P16858 |
| Clone Number | S-240-147 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Zf |
| Predicted Reactivity | Hamster (95%), Mink (95%), Pig (95%), Squirrel (95%),Dog (95%), Cat (95%), Sheep (95%), Quail (95%), Bovine (95%), Chicken (95%) |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:10000-1:50000 | |
| IHC | 1:5000 | |
| ICC | 1:50-1:200 |
Background
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme of about 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules [PMID: 17072346]. GAPDH comprises a polypeptide chain of 335 amino acids. Structural studies identified two regions, namely the glyceraldehyde-3-phosphate catalytic site and the NAD+ binding site, a primary structure known as the Rossmann fold, which is also required for the activity of other dehydrogenases. Many of these roles are dependent on the ability of GAPDH to bind different macromolecules in the cell.
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