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Description
Human TKTL1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against transketolase-like protein 1 (TKTL1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of transketolase-like protein 1 (TKTL1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Transketolase Like Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transketolase-like protein 1 (TKTL1), also known as transketolase 2 (TK2), is an enzyme encoded by the TKTL1 gene. The protein encoded by this gene is a transketolase that acts as a homodimer and catalyzes the conversion of chromobiose 7-phosphate and D-glyceraldehyde 3-phosphate to D-ribose 5-phosphate and D-xylulose 5-phosphate. This reaction links the pentose phosphate pathway with the glycolysis pathway. It catalyzes the transfer of a dicarbon ketol group from a ketose donor to an aldose acceptor via a covalent intermediate with the cofactor thiamine pyrophosphate. Mutations in this gene may be causative of Wernicke-Korsakoff syndrome. Three transcript variants encoding different isoforms have been identified for this gene. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
Based on 7 reviews
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Product Reviews
★★★★★ 1
Love & Hate at the same time
Style: WF-4830, Set: DUAL TRAY (500 sheets)/FAX/ADF/PRINT/COPY/SCAN
I installed, connected to the internet, everything was smooth and the 1st day I printed doc, scanned, sent air etc etc etc, and it was working absolutely great. Next day started asking to perform updates, then it was disconnected to the air computer, I couldn't scan, I connected a cable directly and it didn't work either, becae a nightmare and as last resource, I had to scan through a usb device and then connect to the computer back and forth. Then next days, it was always for updates, downloading more date on my computer as well update the printer, it is like they are spying everything you do with a printer, I just want to use it to print/scan/copy documents or any other info, if it is working just find the 1st day, why these companies begin with harassing the buyer with all these daily updates or connecting hundreds of things to make it work. It was much easier in the past, you just install the program of the printer with a CD and that was it! I had one of those printers before this one and it worked for 12 consecutive years with NO PROBLEMS whatsoever. And the last problem is that IS NOT WORKING AT ALL, I had to stored the printer computer and all my office equipment for 6 months and I plugged in everything and the printer asked for new cartridges, I took them out and they are still full of ink, my fingers completely colored & stained are witnesses & now it says use Epson Support code error 031006 ?!?!?!?!?! There is not a web/ph/any kind of information where do I need to go, I barely use the printer at all, it still has some of the protection plastic and it is already damaged. What a pain. Seriously they should at least add who or where we can ask for help. Now AGAIN buying a new printer because for sure EPSON IS NOT GOING TO HELP ME :((((
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 19, 2024
★★★★★ 5
Beats my HP in every phase for less money
Style: WF-4820, Set: 1. SINGLE TRAY (250 sheets)/FAX/ADF/PRINT/COPY/SCAN
Excellent printer. Scans quickly and quietly. Prints nice quality. Set up was a little problematic but i think mostly from operator error.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 21, 2026
★★★★★ 5
Great printer...I thought
Style: WF-4820, Set: 1. SINGLE TRAY (250 sheets)/FAX/ADF/PRINT/COPY/SCAN
This unit has really great reviews and many from small biz owners who say it works great and lasts. I've had it for a month and I'm really happy with it. It's much better than my last one. If it lasts as so many other reviews say, it's a great purchase.
**UPDATE**
Loved this printer. Only used it a few times. On the 3rd month, I pushed the button to turn on the printer and there was no response at all. I tried everything. Checked all connections and the outlets. It would not turn on at all. I was out of the return window with Amazon, so I called Epson. They told me they are sending a unit to me and to return mine with the box and label in the shipment. But they said they are NOT SENDING A NEW UNIT, BUT A REFURBISHED UNIT! I am not at all happy about this! I purchased a new printer, not a refurbished printer, and I want a new one! So, I'm really unhappy with Epson right now.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 6, 2025
★★★★★ 1
Not a good choice.
Style: WF-4820, Set: 1. SINGLE TRAY (250 sheets)/FAX/ADF/PRINT/COPY/SCAN
I recently purchased this printer with high hopes of a reliable and efficient printing solution. Unfortunately, my experience with this printer has been nothing short of a nightmare.
Connectivity Woes:
The most glaring issue is its abysmal Wi-Fi connectivity. Despite being placed right next to the router, the printer consistently displays a "Poor Wi-Fi Connection" message. This is in stark contrast to all other devices in my home, which work seamlessly with the Wi-Fi network. Even my old laser printer, which is placed close to the same location, functions without any connectivity hiccups. It's baffling how a modern printer can struggle so much to maintain a stable Wi-Fi connection.
Scanning Nightmares:
The scanning functionality of this printer is equally disappointing. The user interface is far from user-friendly, making it a daunting task to set up scanning to email or computer directly from the printer. This is a basic feature that should be intuitive and hassle-free, but instead, it's a convoluted process that requires unnecessary effort and time.
Ineffective Customer Service:
I reached out to customer service in hopes of resolving these issues. While they guided me through a reset process, the solutions they provided were only temporary fixes. The problems resurfaced shortly after, leaving me back at square one. It's frustrating to invest in a product only to find that the customer service solutions are inadequate and short-lived.
Comparative Performance:
What's more perplexing is that my older laser printer, which is from a different brand, continues to perform flawlessly in terms of connectivity and scanning capabilities. This stark contrast highlights the subpar quality of the Epson.
Final Verdict:
I'm deeply disappointed with this printer and regret this purchase immensely. Its inability to maintain a stable Wi-Fi connection, along with the cumbersome scanning setup and unsatisfactory customer service experience, make this printer a headache rather than a helpful device. I strongly advise against considering this printer if you value reliable performance and user-friendly features. Save yourself the frustration and explore more dependable options on the market.
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Reviewed in the United States on August 10, 2023
★★★★★ 5
Easy set up on USB cable. Works as expected on my Windows 11 computer
Style: WF-4820, Set: 1. SINGLE TRAY (250 sheets)/FAX/ADF/PRINT/COPY/SCAN
I got this Epson All-In-One printer for my work. I had been using HP printers for years. I started having problems with paper handling on them. Things like getting a paper jam error when there was not paper jammed in it. I decided to try an Epson for a change. I have had the printer now for three weeks. So far it has worked great. Easy to set up on my Windws 11 computer. Works great for printing and for scanning. I have it set up by USB cable because I don't have reliable wireless internet in that part of the building. No issues with the USB configuration. Ink usage seems good so far. Ink on any printer is expensive, but that was expected. I would recommend this printer.
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Reviewed in the United States on March 28, 2026
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